5ZQV

Crystal Structure of Protein Phosphate 1 complexed with PP1 binding domain of GM

5ZQV の概要

• エントリーDOI: 10.2210/pdb5zqv/pdb
• 分子名称: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, Protein phosphatase 1 regulatory subunit 3A, MANGANESE (II) ION, ... (4 entities in total)
• 機能のキーワード: glycogen metabolism, protein phosphate 1 holoenzyme, hydrolase
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 198277.79

構造登録者

Yu, J.,Xiang, S. (登録日: 2018-04-20, 公開日: 2019-03-13, 最終更新日: 2023-11-22)

主引用文献

Yu, J.,Deng, T.,Xiang, S.
Structural basis for protein phosphatase 1 recruitment by glycogen-targeting subunits.
FEBS J., 285:4646-4659, 2018

PubMed Abstract: The rate-limiting enzymes in glycogen metabolism are subject to regulation by reversible phosphorylation. The glycogen-targeted protein phosphatase 1 (PP1) holoenzyme catalyzes their dephosphorylation. It is composed of a catalytic subunit (PP1C) and a glycogen-targeting subunit (G subunit). To date, seven G subunits have been identified. They all contain an RVxF PP1C-binding motif. The interactions between this motif in the skeletal muscle-specific G and PP1C have been revealed by structural studies. However, whether elements outside of this motif contribute to the interaction with PP1C is not clear. In this study, we found that residues next to the RVxF motif in G also mediate interactions to PP1C and revealed the mechanism of the interaction by structural studies. Sequence analysis revealed that the PP1C-binding region in G is highly conserved among G subunits. Consistently, we found that the equivalent region in the liver-enriched G adopts a similar structure upon binding PP1C. Dephosphorylation experiments indicated that this region and the glycogen-binding region in G cooperate to stimulate PP1C's activity toward glycogen-associated substrates. DATABASES: The structure factors and coordinates for the PP1Cα-G (1-99) and PP1Cα-G (31-105) complexes have been deposited into the Protein Data Bank (http://www.pdb.org), with the accession codes 5ZQV and 5ZT0, respectively.

PubMed: 30422398
DOI: 10.1111/febs.14699

実験手法

X-RAY DIFFRACTION (2.95 Å)