5ZOL

Crystal structure of a three sites mutantion of FSAA complexed with HA and product

5ZOL の概要

• エントリーDOI: 10.2210/pdb5zol/pdb
• 分子名称: Fructose-6-phosphate aldolase 1, 1-hydroxypropan-2-one, (3S,4S)-3,4-dihydroxy-4-(thiophen-2-yl)butan-2-one, ... (7 entities in total)
• 機能のキーワード: fructose-6-phosphate aldolase, donor, acceptor, mutant, flexible, lyase
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 272815.74

構造登録者

Wu, L.,Yang, X.H.,Yu, H.W.,Zhou, J.H. (登録日: 2018-04-13, 公開日: 2019-06-12, 最終更新日: 2024-10-23)

主引用文献

Yang, X.,Wu, L.,Li, A.,Ye, L.,Zhou, J.,Yu, H.
The engineering of decameric d-fructose-6-phosphate aldolase A by combinatorial modulation of inter- and intra-subunit interactions.
Chem.Commun.(Camb.), 56:7561-7564, 2020

PubMed Abstract: The combinatorial modulation of inter- and intra-subunit interactions of decameric d-fructose-6-phosphate aldolase A (FSAA) generated a triple-site variant I31T/Q59T/I195Q FSAA with 27- to 278-fold improvement in activity towards target heteroaromatic aldehydes. X-ray crystallographic data and molecular dynamics simulations ascribed the enhanced activity to the pronounced flexibility of the interface region between subunits, the expanded substrate entrance and binding pocket, and enhanced proton transfer, unambiguously demonstrating the efficiency of this strategy for engineering multimeric enzymes.

PubMed: 32519699
DOI: 10.1039/d0cc02437f

実験手法

X-RAY DIFFRACTION (2.172 Å)