5ZMJ

Crystal structure of the Fab region of a neutralizing fully human antibody against GM-CSF

5ZMJ の概要

• エントリーDOI: 10.2210/pdb5zmj/pdb
• 分子名称: Heavy chain of a Fab fraction of human IgG, Light chain of a Fab fraction of human IgG, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: fab, fully human antibody, gm-csf, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52372.14

構造登録者

Angkawidjaja, C.,Torashima, T. (登録日: 2018-04-04, 公開日: 2019-04-17, 最終更新日: 2024-11-13)

主引用文献

Angkawidjaja, C.,Torashima, T.
Crystal structure of the Fab region of a neutralizing antibody against granulocyte-macrophage colony-stimulating factor.
Acta Crystallogr.,Sect.F, 75:634-639, 2019

PubMed Abstract: An increased level of granulocyte-macrophage colony-stimulating factor has a potential role in the development of autoimmune diseases, and the neutralization of its activity by monoclonal antibodies is a promising therapy for some diseases. Here, the crystal structure of the Fab region of EV1007, a fully human antibody expressed in Chinese hamster ovary cells that was developed from human peripheral blood mononuclear cells, is described. The structure closely resembles that of MB007, which is the Fab region of the same antibody expressed in Escherichia coli [Blech et al. (2012), Biochem. J. 447, 205-215], except at the hinge regions between the immunoglobulin domains and the H3 loop region. This paper presents evidence for the flexibility of the hinge and H3 loop regions of the antibody based on the comparison of two independently solved crystal structures.

PubMed: 31584011
DOI: 10.1107/S2053230X1901238X

実験手法

X-RAY DIFFRACTION (1.81 Å)