5ZLH

Crystal structure of Mn-ProtoporphyrinIX-reconstituted P450BM3

Summary for 5ZLH

• Entry DOI: 10.2210/pdb5zlh/pdb
• Related: 5ZIS
• Descriptor: Bifunctional cytochrome P450/NADPH--P450 reductase, MANGANESE PROTOPORPHYRIN IX (2 entities in total)
• Functional Keywords: cytochrome p450, oxidoreductase
• Biological source: Bacillus megaterium
• Total number of polymer chains: 4
• Total formula weight: 211140.16

Authors

Omura, K.,Aiba, Y.,Onoda, H.,Sugimoto, H.,Shoji, O.,Watanabe, Y. (deposition date: 2018-03-28, release date: 2018-08-15, Last modification date: 2024-03-27)

Primary citation

Omura, K.,Aiba, Y.,Onoda, H.,Stanfield, J.K.,Ariyasu, S.,Sugimoto, H.,Shiro, Y.,Shoji, O.,Watanabe, Y.
Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A.
Chem. Commun. (Camb.), 54:7892-7895, 2018

PubMed Abstract: Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.

PubMed: 29845154
DOI: 10.1039/c8cc02760a

Experimental method

X-RAY DIFFRACTION (3.4 Å)