5ZI1

Crystal structure of Bacillus thuringiensis insecticidal crystal protein Cry7Ca1 (wild type)

Summary for 5ZI1

• Entry DOI: 10.2210/pdb5zi1/pdb
• Descriptor: insecticidal crystal protein Cry7Cal, ACETATE ION (3 entities in total)
• Functional Keywords: insecticidal crystal protein, toxin
• Biological source: Bacillus thuringiensis
• Total number of polymer chains: 2
• Total formula weight: 137660.28

Authors

Jing, X.,Gao, M.,Gong, P. (deposition date: 2018-03-14, release date: 2018-12-26, Last modification date: 2023-11-22)

Primary citation

Jing, X.,Yuan, Y.,Wu, Y.,Wu, D.,Gong, P.,Gao, M.
Crystal structure of Bacillus thuringiensis Cry7Ca1 toxin active against Locusta migratoria manilensis.
Protein Sci., 28:609-619, 2019

PubMed Abstract: Insecticidal crystal (Cry) proteins produced by Bacillus thuringiensis (Bt) are widely used as environmentally friendly insecticides. As the only known Cry protein with insecticidal activity against Locusta migratoria manilensis, a locust subspecies that causes extensive destruction of crops, the Cry7Ca1 protein from Bt strain BTH-13 identified in our previous study is of particular interest to locust prevention and control. However, the three-dimensional structure of Cry7Ca1 toxin (the active form of the Cry7Ca1 protein) and the mechanisms of the Cry7Ca1 insecticidal specificity remain largely elusive. Here, we report a 2.3 Å crystal structure of the Cry7Ca1 toxin and carry out a systematic comparison of all available Cry toxins structures. A cluster of six loops in Cry toxin domain II, named Apex here, are the most variable structural elements and were documented to contribute in insecticidal specificity. The Cry7Ca1 toxin Apex loops are different from those of other Cry toxins in length, conformation, and sequence. Electrostatic potential analysis further revealed that Cry7Ca1 is the only structure-available Cry toxin that does not have a high contrast of surface electrostatic potentials in the Apex. We further suggest that the L1/L2 loops in the center of the Cry7Ca1 Apex may be worthy of attention in future efforts to unravel the Cry7Ca1 insecticidal specificity as they exhibit unique features not found in the corresponding regions of other Cry toxins. Our work highlights the uniqueness of the Apex in the Cry7Ca1 toxin and may assist exploration of the insecticidal mechanism of the Cry7Ca1 against Locusta migratoria manilensis.

PubMed: 30506755
DOI: 10.1002/pro.3561

Experimental method

X-RAY DIFFRACTION (2.3 Å)