5ZEN

Crystal structure of human topoisomerase II beta in complex with DNA: a new quaternary conformation showing opening of the protein-linked DNA-gate

5ZEN の概要

• エントリーDOI: 10.2210/pdb5zen/pdb
• 分子名称: DNA topoisomerase 2-beta, DNA (5'-D(P*AP*GP*CP*CP*GP*AP*GP*C)-3'), DNA (5'-D(P*AP*GP*CP*TP*CP*GP*GP*CP*T)-3'), ... (5 entities in total)
• 機能のキーワード: type ii topoisomerase, cleavage complex, dna-gate, isomerase-dna complex, isomerase/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 97206.66

構造登録者

Chen, S.F.,Wang, Y.R.,Chan, N.L. (登録日: 2018-02-27, 公開日: 2018-08-08, 最終更新日: 2023-11-22)

主引用文献

Chen, S.F.,Huang, N.L.,Lin, J.H.,Wu, C.C.,Wang, Y.R.,Yu, Y.J.,Gilson, M.K.,Chan, N.L.
Structural insights into the gating of DNA passage by the topoisomerase II DNA-gate.
Nat Commun, 9:3085-3085, 2018

PubMed Abstract: Type IIA topoisomerases (Top2s) manipulate the handedness of DNA crossovers by introducing a transient and protein-linked double-strand break in one DNA duplex, termed the DNA-gate, whose opening allows another DNA segment to be transported through to change the DNA topology. Despite the central importance of this gate-opening event to Top2 function, the DNA-gate in all reported structures of Top2-DNA complexes is in the closed state. Here we present the crystal structure of a human Top2 DNA-gate in an open conformation, which not only reveals structural characteristics of its DNA-conducting path, but also uncovers unexpected yet functionally significant conformational changes associated with gate-opening. This structure further implicates Top2's preference for a left-handed DNA braid and allows the construction of a model representing the initial entry of another DNA duplex into the DNA-gate. Steered molecular dynamics calculations suggests the Top2-catalyzed DNA passage may be achieved by a rocker-switch-type movement of the DNA-gate.

PubMed: 30082834
DOI: 10.1038/s41467-018-05406-y

実験手法

X-RAY DIFFRACTION (2.75 Å)