5ZCN

Solution NMR structure of a new lasso peptide brevunsin

Summary for 5ZCN

• Entry DOI: 10.2210/pdb5zcn/pdb
• NMR Information: BMRB: 36167
• Descriptor: brevunsin (1 entity in total)
• Functional Keywords: lasso peptide, sphingomonas subterranea, brevunsin, unknown function
• Biological source: Brevundimonas diminuta
• Total number of polymer chains: 1
• Total formula weight: 2253.47

Authors

Hemmi, H.,Kodani, S.,Miyake, Y.,Kaweewan, I.,Nakagawa, H. (deposition date: 2018-02-19, release date: 2018-10-17, Last modification date: 2024-10-09)

Primary citation

Kodani, S.,Hemmi, H.,Miyake, Y.,Kaweewan, I.,Nakagawa, H.
Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea
J. Ind. Microbiol. Biotechnol., 45:983-992, 2018

PubMed Abstract: A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes.

PubMed: 30191430
DOI: 10.1007/s10295-018-2077-6

Experimental method

SOLUTION NMR