5Z8A

Crystal structure of GenB1 from Micromonospora echinospora in complex with JI-20A and PLP (external aldimine)

Summary for 5Z8A

DescriptorC-6' aminotransferase, PYRIDOXAL-5'-PHOSPHATE, (2~{R},3~{R},4~{R},5~{R})-2-[(1~{S},2~{S},3~{R},4~{S},6~{R})-3-[(2~{R},3~{R},4~{R},5~{S},6~{R})-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol, ... (4 entities in total)
Functional Keywordsaminotransferase, plp-binding, transferase
Biological sourceMicromonospora echinospora (Micromonospora purpurea)
Total number of polymer chains2
Total molecular weight95806.18
Authors
Hong, S.K.,Cha, S.S. (deposition date: 2018-01-31, release date: 2019-01-16, Last modification date: 2019-01-30)
Primary citation
Ban, Y.H.,Song, M.C.,Hwang, J.Y.,Shin, H.L.,Kim, H.J.,Hong, S.K.,Lee, N.J.,Park, J.W.,Cha, S.S.,Liu, H.W.,Yoon, Y.J.
Complete reconstitution of the diverse pathways of gentamicin B biosynthesis
Nat. Chem. Biol., 2019
PubMed: 30643280 (PDB entries with the same primary citation)
DOI: 10.1038/s41589-018-0203-4
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.398 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.1941 0.2% 1.4% 2.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5z8a
no rotation
Molmil generated image of 5z8a
rotated about x axis by 90°
Molmil generated image of 5z8a
rotated about y axis by 90°
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