5Z8A
Crystal structure of GenB1 from Micromonospora echinospora in complex with JI-20A and PLP (external aldimine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.765, 88.687, 116.849 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.389 - 1.398 |
R-factor | 0.1719 |
Rwork | 0.172 |
R-free | 0.19390 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.754 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2474) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.398 | 1.400 |
Rmerge | 0.052 | 0.325 |
Rpim | 0.016 | 0.158 |
Number of reflections | 144352 | 8225 |
<I/σ(I)> | 37.1 | |
Completeness [%] | 92.0 | |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 295 | magnesium formate, PEG3350 |