5Z56

cryo-EM structure of a human activated spliceosome (mature Bact) at 5.1 angstrom.

これはPDB形式変換不可エントリーです。

5Z56 の概要

• エントリーDOI: 10.2210/pdb5z56/pdb
• 関連するPDBエントリー: 5Z57 5Z58
• EMDBエントリー: 6889 6890 6891
• 分子名称: Pre-mRNA-processing-splicing factor 8, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein E, ... (53 entities in total)
• 機能のキーワード: human activated spliceosome, mature bact, pre-mrna splicing, splicing
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 59
• 化学式量合計: 3602989.39

構造登録者

Zhang, X.,Yan, C.,Zhan, X.,Li, L.,Lei, J.,Shi, Y. (登録日: 2018-01-17, 公開日: 2018-09-19, 最終更新日: 2024-11-13)

主引用文献

Zhang, X.,Yan, C.,Zhan, X.,Li, L.,Lei, J.,Shi, Y.
Structure of the human activated spliceosome in three conformational states.
Cell Res., 28:307-322, 2018

PubMed Abstract: During each cycle of pre-mRNA splicing, the pre-catalytic spliceosome (B complex) is converted into the activated spliceosome (B complex), which has a well-formed active site but cannot proceed to the branching reaction. Here, we present the cryo-EM structure of the human B complex in three distinct conformational states. The EM map allows atomic modeling of nearly all protein components of the U2 small nuclear ribonucleoprotein (snRNP), including three of the SF3a complex and seven of the SF3b complex. The structure of the human B complex contains 52 proteins, U2, U5, and U6 small nuclear RNA (snRNA), and a pre-mRNA. Three distinct conformations have been captured, representing the early, mature, and late states of the human B complex. These complexes differ in the orientation of the Switch loop of Prp8, the splicing factors RNF113A and NY-CO-10, and most components of the NineTeen complex (NTC) and the NTC-related complex. Analysis of these three complexes and comparison with the B and C complexes reveal an ordered flux of components in the B-to-B and the B-to-B transitions, which ultimately prime the active site for the branching reaction.

PubMed: 29360106
DOI: 10.1038/cr.2018.14

実験手法

ELECTRON MICROSCOPY (5.1 Å)