5Z3G
Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)
Summary for 5Z3G
| Entry DOI | 10.2210/pdb5z3g/pdb |
| EMDB information | 6878 |
| Descriptor | 25S rRNA, 60S ribosomal protein L7-A, 60S ribosomal protein L8-A, ... (35 entities in total) |
| Functional Keywords | ribosome, pre-60s, pre-ribosome, protein-rna complex |
| Biological source | Saccharomyces cerevisiae (Baker's yeast) More |
| Total number of polymer chains | 35 |
| Total formula weight | 2136093.15 |
| Authors | |
| Primary citation | Zhou, D.,Zhu, X.,Zheng, S.,Tan, D.,Dong, M.Q.,Ye, K. Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate. Protein Cell, 10:120-130, 2019 Cited by PubMed Abstract: Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 Å resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits. PubMed: 29557065DOI: 10.1007/s13238-018-0526-7 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.65 Å) |
Structure validation
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