5Z3G

Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)

5Z3G の概要

• エントリーDOI: 10.2210/pdb5z3g/pdb
• EMDBエントリー: 6878
• 分子名称: 25S rRNA, 60S ribosomal protein L7-A, 60S ribosomal protein L8-A, ... (35 entities in total)
• 機能のキーワード: ribosome, pre-60s, pre-ribosome, protein-rna complex
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 2136093.15

構造登録者

Zhu, X.,Zhou, D.,Ye, K. (登録日: 2018-01-06, 公開日: 2018-04-11, 最終更新日: 2024-11-06)

主引用文献

Zhou, D.,Zhu, X.,Zheng, S.,Tan, D.,Dong, M.Q.,Ye, K.
Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.
Protein Cell, 10:120-130, 2019

PubMed Abstract: Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 Å resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits.

PubMed: 29557065
DOI: 10.1007/s13238-018-0526-7

実験手法

ELECTRON MICROSCOPY (3.65 Å)