5Z2U

ThDP-Mn2+ complex of R395A variant of EcMenD soaked with 2-ketoglutarate for 5 min

Summary for 5Z2U

• Entry DOI: 10.2210/pdb5z2u/pdb
• Descriptor: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase, (4S)-4-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3lambda~5~-thiazol-2-yl}-4-hydroxybutanoic acid, MAGNESIUM ION, ... (8 entities in total)
• Functional Keywords: complex, transferase
• Biological source: Escherichia coli (strain K12)
• Total number of polymer chains: 8
• Total formula weight: 496075.67

Authors

Qin, M.M.,Guo, Z.H. (deposition date: 2018-01-04, release date: 2018-11-28, Last modification date: 2023-11-22)

• Primary citation: Qin, M.M.,Song, H.G.,Dai, X.,Chen, Y.Z.,Guo, Z.H.; Two active site arginines are critical determinants of substrate binding and catalysis in MenD: a thiamine-dependent enzyme in menaquinone biosynthesis.; Biochem. J., 475:3651-3667, 2018; PubMed: 30341164; DOI: 10.1042/BCJ20180548

Experimental method

X-RAY DIFFRACTION (2.35 Å)