5YZ0

Cryo-EM Structure of human ATR-ATRIP complex

Summary for 5YZ0

• Entry DOI: 10.2210/pdb5yz0/pdb
• EMDB information: 6862
• Descriptor: Serine/threonine-protein kinase ATR, ATR-interacting protein (2 entities in total)
• Functional Keywords: cryo-em, atr-atrip, dna damnage response, cell cycle
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 775394.89

Authors

Rao, Q.,Liu, M.,Tian, Y.,Wu, Z.,Wang, H.,Wang, J.,Xu, Y. (deposition date: 2017-12-11, release date: 2018-01-31, Last modification date: 2024-10-16)

Primary citation

Rao, Q.,Liu, M.,Tian, Y.,Wu, Z.,Hao, Y.,Song, L.,Qin, Z.,Ding, C.,Wang, H.W.,Wang, J.,Xu, Y.
Cryo-EM structure of human ATR-ATRIP complex.
Cell Res., 28:143-156, 2018

PubMed Abstract: ATR (ataxia telangiectasia-mutated and Rad3-related) protein kinase and ATRIP (ATR-interacting protein) form a complex and play a critical role in response to replication stress and DNA damage. Here, we determined the cryo-electron microscopy (EM) structure of the human ATR-ATRIP complex at 4.7 Å resolution and built an atomic model of the C-terminal catalytic core of ATR (residues 1 521-2 644) at 3.9 Å resolution. The complex adopts a hollow "heart" shape, consisting of two ATR monomers in distinct conformations. The EM map for ATRIP reveals 14 HEAT repeats in an extended "S" shape. The conformational flexibility of ATR allows ATRIP to properly lock the N-termini of the two ATR monomers to favor ATR-ATRIP complex formation and functional diversity. The isolated "head-head" and "tail-tail" each adopts a pseudo 2-fold symmetry. The catalytic pockets face outward and substrate access is not restricted by inhibitory elements. Our studies provide a structural basis for understanding the assembly of the ATR-ATRIP complex and a framework for characterizing ATR-mediated DNA repair pathways.

PubMed: 29271416
DOI: 10.1038/cr.2017.158

Experimental method

ELECTRON MICROSCOPY (4.7 Å)