5YTH

Structure of large fragment of DNA Polymerase I from Thermus aquaticus Host-Guest complex with the unnatural base M-fC pair with dG

Summary for 5YTH

• Entry DOI: 10.2210/pdb5yth/pdb
• Descriptor: DNA polymerase I, thermostable, DNA (5'-D(*GP*AP*CP*CP*GP*CP*GP*GP*CP*GP*CP*(DOC))-3'), DNA (5'-D(*AP*AP*AP*CP*GP*GP*CP*GP*CP*CP*GP*(92F)P*GP*GP*TP*C)-3'), ... (7 entities in total)
• Functional Keywords: dna, replication, transferase-dna complex, transferase/dna
• Biological source: Thermus aquaticus; More
• Total number of polymer chains: 3
• Total formula weight: 70132.38

Authors

Zeng, H.,Mondal, M.,Song, R.Y.,Zhang, J.,Xia, B.,Gao, Y.Q.,Yi, C.Q. (deposition date: 2017-11-17, release date: 2018-11-21, Last modification date: 2023-11-22)

Primary citation

Zeng, H.,Mondal, M.,Song, R.,Zhang, J.,Xia, B.,Liu, M.,Zhu, C.,He, B.,Gao, Y.Q.,Yi, C.
Unnatural Cytosine Bases Recognized as Thymines by DNA Polymerases by the Formation of the Watson-Crick Geometry.
Angew. Chem. Int. Ed. Engl., 58:130-133, 2019

PubMed Abstract: The emergence of unnatural DNA bases provides opportunities to demystify the mechanisms by which DNA polymerases faithfully decode chemical information on the template. It was previously shown that two unnatural cytosine bases (termed "M-fC" and "I-fC"), which are chemical labeling adducts of the epigenetic base 5-formylcytosine, can induce C-to-T transition during DNA amplification. However, how DNA polymerases recognize such unnatural cytosine bases remains enigmatic. Herein, crystal structures of unnatural cytosine bases pairing to dA/dG in the KlenTaq polymerase-host-guest complex system and pairing to dATP in the KlenTaq polymerase active site were determined. Both M-fC and I-fC base pair with dA/dATP, but not with dG, in a Watson-Crick geometry. This study reveals that the formation of the Watson-Crick geometry, which may be enabled by the A-rule, is important for the recognition of unnatural cytosines.

PubMed: 30407705
DOI: 10.1002/anie.201807845

Experimental method

X-RAY DIFFRACTION (2.53 Å)