5YSZ

transcriptional regulator CelR-cellobiose complex

Summary for 5YSZ

• Entry DOI: 10.2210/pdb5ysz/pdb
• Related PRD ID: PRD_900005
• Descriptor: Transcriptional regulator, LacI family, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, GLYCEROL, ... (4 entities in total)
• Functional Keywords: transcriptional regulator, cellulase gene regulator, laci/galr family, cellobiose binding protein, complex, transcription
• Biological source: Thermobifida fusca YX
• Total number of polymer chains: 1
• Total formula weight: 39512.57

Authors

Fu, Y.,Yeom, S.Y.,Lee, D.H.,Lee, S.G. (deposition date: 2017-11-16, release date: 2018-08-22, Last modification date: 2023-11-22)

Primary citation

Fu, Y.,Yeom, S.J.,Kwon, K.K.,Hwang, J.,Kim, H.,Woo, E.J.,Lee, D.H.,Lee, S.G.
Structural and functional analyses of the cellulase transcription regulator CelR
FEBS Lett., 592:2776-2785, 2018

PubMed Abstract: CelR is a transcriptional regulator that controls the expression of cellulases catalyzing cellulose hydrolysis. However, the structural mechanism of its regulation has remained unclear. Here, we report the first structure of CelR, in this case with cellobiose bound. CelR consists of a DNA-binding domain (DBD) and a regulatory domain (RD), and homodimerizes with each monomer bound to cellobiose. A hinge region (HR) in CelR connects the DBD with the RD, and Leu59 in the HR acts as a 'leucine lever' that transduces a transcriptional activation signal. Furthermore, an α4 helix mediates the ligand-binding signal for transcriptional activation. Tyr84 and Gln301 can potentially alter the ligand specificity of CelR. This study provides a pivotal step toward understanding transcription of the cellulases.

PubMed: 30062758
DOI: 10.1002/1873-3468.13206

Experimental method

X-RAY DIFFRACTION (1.631 Å)