5YP2

Crystal structure of dipeptidyl peptidase IV (DPP IV) with DPP4 inhibitor from Pseudoxanthomonas mexicana WO24

5YP2 の概要

• エントリーDOI: 10.2210/pdb5yp2/pdb
• 関連するPDBエントリー: 5YP1 5YP3 5YP4
• 分子名称: Dipeptidyl aminopeptidase 4, (2S,5R)-1-[2-[[1-(hydroxymethyl)cyclopentyl]amino]ethanoyl]pyrrolidine-2,5-dicarbonitrile, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: dap iv, clan sc s9, peptidase, dpp4, dpp8, dpp9, hydrolase
• 由来する生物種: Pseudoxanthomonas mexicana (Pseudomonas sp. WO24)
• 細胞内の位置: Isoform 2: Cytoplasm . Isoform 1: Periplasm : Q6F3I7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 165579.95

構造登録者

Roppongi, S.,Suzuki, Y.,Tateoka, C.,Fuimoto, M.,Morisawa, S.,Iizuka, I.,Nakamura, A.,Honma, N.,Shida, Y.,Ogasawara, W.,Tanaka, N.,Sakamoto, Y.,Nonaka, T. (登録日: 2017-11-01, 公開日: 2018-02-21, 最終更新日: 2023-11-22)

主引用文献

Roppongi, S.,Suzuki, Y.,Tateoka, C.,Fujimoto, M.,Morisawa, S.,Iizuka, I.,Nakamura, A.,Honma, N.,Shida, Y.,Ogasawara, W.,Tanaka, N.,Sakamoto, Y.,Nonaka, T.
Crystal structures of a bacterial dipeptidyl peptidase IV reveal a novel substrate recognition mechanism distinct from that of mammalian orthologues.
Sci Rep, 8:2714-2714, 2018

PubMed Abstract: Dipeptidyl peptidase IV (DPP IV, DPP4, or DAP IV) preferentially cleaves substrate peptides with Pro or Ala at the P1 position. The substrate recognition mechanism has been fully elucidated for mammalian DPP IV by crystal structure analyses but not for bacterial orthologues. Here, we report the crystal structures of a bacterial DPP IV (PmDAP IV) in its free form and in complexes with two kinds of dipeptides as well as with a non-peptidyl inhibitor at 1.90 to 2.47 Å resolution. Acyl-enzyme intermediates were observed for the dipeptide complexes of PmDAP IV, whereas tetrahedral intermediates were reported for the oligopeptide complexes of mammalian DPP IVs. This variation reflects the different structural environments of the active site Arg residues, which are involved in the recognition of a substrate carbonyl group, of mammalian and bacterial enzymes. A phylogenetic analysis revealed that PmDAP IV is a closer relative of dipeptidyl peptidases 8 and 9 (DPP8 and DPP9, DPP IV-family enzymes) than DPP IV. These results provide new insights into the substrate recognition mechanism of bacterial DAP IVs and may assist in the development of selective inhibitors for DAP IVs from pathogenic asaccharolytic bacteria, which utilise proteins or peptides as an energy source.

PubMed: 29426867
DOI: 10.1038/s41598-018-21056-y

実験手法

X-RAY DIFFRACTION (2.13 Å)