5YKI

Crystal structure of the engineered nine-repeat PUF domain in complex with cognate 9nt-RNA

Summary for 5YKI

• Entry DOI: 10.2210/pdb5yki/pdb
• Related: 5YKH
• Descriptor: Pumilio homolog 1, RNA (5'-R(*UP*GP*UP*UP*GP*UP*AP*UP*A)-3') (3 entities in total)
• Functional Keywords: puf repeats, engineered protein, rna recognition, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 50896.68

Authors

Zhao, Y.Y.,Wang, J.,Li, H.T.,Wang, Z.X.,Wu, J.W. (deposition date: 2017-10-14, release date: 2018-03-14, Last modification date: 2023-11-22)

• Primary citation: Zhao, Y.Y.,Mao, M.W.,Zhang, W.J.,Wang, J.,Li, H.T.,Yang, Y.,Wang, Z.,Wu, J.W.; Expanding RNA binding specificity and affinity of engineered PUF domains.; Nucleic Acids Res., 46:4771-4782, 2018; PubMed: 29490074; DOI: 10.1093/nar/gky134

Experimental method

X-RAY DIFFRACTION (2.25 Å)