5YKH

Crystal structure of the engineered nine-repeat PUF domain

5YKH の概要

• エントリーDOI: 10.2210/pdb5ykh/pdb
• 分子名称: Pumilio homolog 1, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: puf repeats, engineered protein, rna recognition, rna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48346.89

構造登録者

Zhao, Y.Y.,Wang, J.,Li, H.T.,Wang, Z.X.,Wu, J.W. (登録日: 2017-10-14, 公開日: 2018-03-14, 最終更新日: 2023-11-22)

主引用文献

Zhao, Y.Y.,Mao, M.W.,Zhang, W.J.,Wang, J.,Li, H.T.,Yang, Y.,Wang, Z.,Wu, J.W.
Expanding RNA binding specificity and affinity of engineered PUF domains.
Nucleic Acids Res., 46:4771-4782, 2018

PubMed Abstract: Specific manipulation of RNA is necessary for the research in biotechnology and medicine. The RNA-binding domains of Pumilio/fem-3 mRNA binding factors (PUF domains) are programmable RNA binding scaffolds used to engineer artificial proteins that specifically modulate RNAs. However, the native PUF domains generally recognize 8-nt RNAs, limiting their applications. Here, we modify the PUF domain of human Pumilio1 to engineer PUFs that recognize RNA targets of different length. The engineered PUFs bind to their RNA targets specifically and PUFs with more repeats have higher binding affinity than the canonical eight-repeat domains; however, the binding affinity reaches the peak at those with 9 and 10 repeats. Structural analysis on PUF with nine repeats reveals a higher degree of curvature, and the RNA binding unexpectedly and dramatically opens the curved structure. Investigation of the residues positioned in between two RNA bases demonstrates that tyrosine and arginine have favored stacking interactions. Further tests on the availability of the engineered PUFs in vitro and in splicing function assays indicate that our engineered PUFs bind RNA targets with high affinity in a programmable way.

PubMed: 29490074
DOI: 10.1093/nar/gky134

実験手法

X-RAY DIFFRACTION (2.457 Å)