5YIP

Crystal Structure of AnkG LIR/GABARAPL1 complex

5YIP の概要

• エントリーDOI: 10.2210/pdb5yip/pdb
• 分子名称: Gamma-aminobutyric acid receptor-associated protein-like 1, Ankyrin-3, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: autophagy, signaling protein
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17654.75

構造登録者

Li, J.,Zhu, R.,Chen, K.,Zheng, H.,Yuan, C.,Zhang, H.,Wang, C.,Zhang, M. (登録日: 2017-10-06, 公開日: 2018-05-23, 最終更新日: 2023-11-22)

主引用文献

Li, J.,Zhu, R.,Chen, K.,Zheng, H.,Zhao, H.,Yuan, C.,Zhang, H.,Wang, C.,Zhang, M.
Potent and specific Atg8-targeting autophagy inhibitory peptides from giant ankyrins.
Nat. Chem. Biol., 14:778-787, 2018

PubMed Abstract: The mammalian Atg8 family proteins are central drivers of autophagy and contain six members, classified into the LC3 and GABARAP subfamilies. Due to their high sequence similarity and consequent functional overlaps, it is difficult to delineate specific functions of Atg8 proteins in autophagy. Here we discover a super-strong GABARAP-selective inhibitory peptide harbored in 270/480 kDa ankyrin-G and a super-potent pan-Atg8 inhibitory peptide from 440 kDa ankyrin-B. Structural studies elucidate the mechanism governing the Atg8 binding potency and selectivity of the peptides, reveal a general Atg8-binding sequence motif, and allow development of a more GABARAP-selective inhibitory peptide. These peptides effectively blocked autophagy when expressed in cultured cells. Expression of these ankyrin-derived peptides in Caenorhabditis elegans also inhibited autophagy, causing accumulation of the p62 homolog SQST-1, delayed development and shortened life span. Thus, these genetically encodable autophagy inhibitory peptides can be used to occlude autophagy spatiotemporally in living animals.

PubMed: 29867141
DOI: 10.1038/s41589-018-0082-8

実験手法

X-RAY DIFFRACTION (1.85 Å)