5YFC

Crystal structure of a new DPP III family member

Summary for 5YFC

• Entry DOI: 10.2210/pdb5yfc/pdb
• Descriptor: Dipeptidyl peptidase 3, SULFATE ION, CHLORIDE ION, ... (6 entities in total)
• Functional Keywords: dipeptidyl peptidase iii, aflatoxin-oxidase, hydrolase
• Biological source: Armillaria tabescens (Ringless honey mushroom)
• Total number of polymer chains: 2
• Total formula weight: 158242.18

Authors

Xu, T.,Liu, J. (deposition date: 2017-09-20, release date: 2018-01-03, Last modification date: 2024-03-27)

Primary citation

Xu, T.,Xie, C.,Yao, D.,Zhou, C.Z.,Liu, J.
Crystal structures of Aflatoxin-oxidase from Armillariella tabescens reveal a dual activity enzyme.
Biochem. Biophys. Res. Commun., 494:621-625, 2017

PubMed Abstract: Aflatoxin-oxidase (AFO), a newly discovered oxidase isolated from Armillariella tabescens, was reported to perform aflatoxin B1 (AFB1) detoxification through breaking the bisfuran ring of AFB1. However, based on sequence alignment, we found that AFO shares high sequence identities with dipeptidyl peptidase III (DPP III) family members. To understand the functions of AFO, we determined its crystal structures in the absence and presence of zinc, copper ion, and employed HPLC to test if AFO could cleave the substrates of DPP III. Our structures reveal that AFO contains the classic DPP III activity center and the HPLC results further confirm that AFO possesses the dipeptidyl peptidase activity. Therefore, AFO should belong to DPP III family. Interestingly, unlike reported classic DPP III structure that has a large domain movement upon substrate binding, the AFO structures all adopt the closed conformation, independent of substrate binding. This conformation characteristic of AFO may be related to its enzyme activities. Taken together, our results demonstrate that AFO is a dual activity enzyme with both aflatoxin-oxidase and dipeptidyl peptidase activities and its unique conformation feature expands our understanding on the mode of reaction for this enzyme family.

PubMed: 29050944
DOI: 10.1016/j.bbrc.2017.10.077

Experimental method

X-RAY DIFFRACTION (1.76 Å)