5Y85

Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme

5Y85 の概要

• エントリーDOI: 10.2210/pdb5y85/pdb
• 分子名称: RNA (5'-R(P*AP*CP*CP*CP*GP*CP*AP*AP*GP*GP*CP*CP*GP*AP*CP*GP*GP*C)-3'), DNA/RNA (50-MER), MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: twister-sister, riboyzme, catalysis, self-cleaving, rna
• 由来する生物種: metagenome; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 44376.16

構造登録者

Zheng, L.,Micura, R.L.,Ren, A. (登録日: 2017-08-18, 公開日: 2017-11-22, 最終更新日: 2024-03-27)

主引用文献

Zheng, L.,Mairhofer, E.,Teplova, M.,Zhang, Y.,Ma, J.,Patel, D.J.,Micura, R.,Ren, A.
Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme
Nat Commun, 8:1180-1180, 2017

PubMed Abstract: Here we report on the crystal structure and cleavage assays of a four-way junctional twister-sister self-cleaving ribozyme. Notably, 11 conserved spatially separated loop nucleotides are brought into close proximity at the ribozyme core through long-range interactions mediated by hydrated Mg cations. The C62-A63 step at the cleavage site adopts a splayed-apart orientation, with flexible C62 directed outwards, whereas A63 is directed inwards and anchored by stacking and hydrogen-bonding interactions. Structure-guided studies of key base, sugar, and phosphate mutations in the twister-sister ribozyme, suggest contributions to the cleavage chemistry from interactions between a guanine at the active site and the non-bridging oxygen of the scissile phosphate, a feature found previously also for the related twister ribozyme. Our four-way junctional pre-catalytic structure differs significantly in the alignment at the cleavage step (splayed-apart vs. base-stacked) and surrounding residues and hydrated Mg ions relative to a reported three-way junctional pre-catalytic structure of the twister-sister ribozyme.

PubMed: 29081514
DOI: 10.1038/s41467-017-01276-y

実験手法

X-RAY DIFFRACTION (2.001 Å)