5XUR

Crystal Structure of Rv2466c C22S Mutant

5XUR の概要

• エントリーDOI: 10.2210/pdb5xur/pdb
• 分子名称: Thioredoxin-like reductase Rv2466c, 1,2-ETHANEDIOL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: thioredoxin-like, oxidoreductase
• 由来する生物種: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
• 細胞内の位置: Cytoplasm : O53193
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 96575.87

構造登録者

Zhang, X.,Li, H. (登録日: 2017-06-25, 公開日: 2018-03-14, 最終更新日: 2023-11-22)

主引用文献

Negri, A.,Javidnia, P.,Mu, R.,Zhang, X.,Vendome, J.,Gold, B.,Roberts, J.,Barman, D.,Ioerger, T.,Sacchettini, J.C.,Jiang, X.,Burns-Huang, K.,Warrier, T.,Ling, Y.,Warren, J.D.,Oren, D.A.,Beuming, T.,Wang, H.,Wu, J.,Li, H.,Rhee, K.Y.,Nathan, C.F.,Liu, G.,Somersan-Karakaya, S.
Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis.
ACS Infect Dis, 4:771-787, 2018

PubMed Abstract: The success of Mycobacterium tuberculosis (Mtb) as a pathogen depends on the redundant and complex mechanisms it has evolved for resisting nitrosative and oxidative stresses inflicted by host immunity. Improving our understanding of these defense pathways can reveal vulnerable points in Mtb pathogenesis. In this study, we combined genetic, structural, computational, biochemical, and biophysical approaches to identify a novel enzyme class represented by Rv2466c. We show that Rv2466c is a mycothiol-dependent nitroreductase of Mtb and can reduce the nitro group of a novel mycobactericidal compound using mycothiol as a cofactor. In addition to its function as a nitroreductase, Rv2466c confers partial protection to menadione stress.

PubMed: 29465985
DOI: 10.1021/acsinfecdis.7b00111

実験手法

X-RAY DIFFRACTION (1.996 Å)