5XQ1

Structural basis of kindlin-mediated integrin recognition and activation

Summary for 5XQ1

• Entry DOI: 10.2210/pdb5xq1/pdb
• Descriptor: Fermitin family homolog 2,Integrin beta-3 (2 entities in total)
• Functional Keywords: integrin binding, multi-domain containing protein, signaling protein
• Biological source: Mus musculus (Mouse); More
• Cellular location: Cell membrane ; Single-pass type I membrane protein : O54890
• Total number of polymer chains: 2
• Total formula weight: 112269.29

Authors

Li, H.,Yang, H.,Sun, K.,Zhang, Z.,Yu, C.,Wei, Z. (deposition date: 2017-06-05, release date: 2017-07-26, Last modification date: 2023-11-22)

Primary citation

Li, H.,Deng, Y.,Sun, K.,Yang, H.,Liu, J.,Wang, M.,Zhang, Z.,Lin, J.,Wu, C.,Wei, Z.,Yu, C.
Structural basis of kindlin-mediated integrin recognition and activation
Proc. Natl. Acad. Sci. U.S.A., 114:9349-9354, 2017

PubMed Abstract: Kindlins and talins are integrin-binding proteins that are critically involved in integrin activation, an essential process for many fundamental cellular activities including cell-matrix adhesion, migration, and proliferation. As FERM-domain-containing proteins, talins and kindlins, respectively, bind different regions of β-integrin cytoplasmic tails. However, compared with the extensively studied talin, little is known about how kindlins specifically interact with integrins and synergistically enhance their activation by talins. Here, we determined crystal structures of kindlin2 in the apo-form and the β1- and β3-integrin bound forms. The apo-structure shows an overall architecture distinct from talins. The complex structures reveal a unique integrin recognition mode of kindlins, which combines two binding motifs to provide specificity that is essential for integrin activation and signaling. Strikingly, our structures uncover an unexpected dimer formation of kindlins. Interrupting dimer formation impairs kindlin-mediated integrin activation. Collectively, the structural, biochemical, and cellular results provide mechanistic explanations that account for the effects of kindlins on integrin activation as well as for how kindlin mutations found in patients with Kindler syndrome and leukocyte-adhesion deficiency may impact integrin-mediated processes.

PubMed: 28739949
DOI: 10.1073/pnas.1703064114

Experimental method

X-RAY DIFFRACTION (2.954 Å)