5XPD

Sugar transporter of AtSWEET13 in inward-facing state with a substrate analog

5XPD の概要

• エントリーDOI: 10.2210/pdb5xpd/pdb
• 分子名称: sugar transporter, 2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE (2 entities in total)
• 機能のキーワード: sweets sugar transporter complex, transport protein
• 由来する生物種: Arabidopsis thaliana
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33313.86

構造登録者

Han, L.,Zhang, X.J. (登録日: 2017-06-01, 公開日: 2017-09-13, 最終更新日: 2023-11-22)

主引用文献

Han, L.,Zhu, Y.P.,Liu, M.,Zhou, Y.,Lu, G.Y.,Lan, L.,Wang, X.P.,Zhao, Y.F.,Zhang, X.C.
Molecular mechanism of substrate recognition and transport by the AtSWEET13 sugar transporter
Proc. Natl. Acad. Sci. U.S.A., 114:10089-10094, 2017

PubMed Abstract: Sugar Will Eventually be Exported Transporters (SWEETs) are recently identified sugar transporters that can discriminate and transport di- or monosaccharides across a membrane following the concentration gradient. SWEETs play key roles in plant biological processes, such as pollen nutrition, nectar secretion, seed filling, and phloem loading. SWEET13 from (AtSWEET13) is an important sucrose transporter in pollen development. Here, we report the 2.8-Å resolution crystal structure of AtSWEET13 in the inward-facing conformation with a substrate analog, 2'-deoxycytidine 5'-monophosphate, bound in the central cavity. In addition, based on the results of an in-cell transport activity assay and single-molecule Förster resonance energy transfer analysis, we suggest a mechanism for substrate selectivity based on the size of the substrate-binding pocket. Furthermore, AtSWEET13 appears to form a higher order structure presumably related to its function.

PubMed: 28878024
DOI: 10.1073/pnas.1709241114

実験手法

X-RAY DIFFRACTION (2.788 Å)