5XKU

Crystal structure of hemagglutinin globular head from an H7N9 influenza virus in complex with a neutralizing antibody HNIgGA6

Summary for 5XKU

• Entry DOI: 10.2210/pdb5xku/pdb
• Descriptor: Influenza a virus, HNIgGA6 light chain, HNIgGA6 heavy chain, ... (5 entities in total)
• Functional Keywords: hemagglutinin, complex, antibody, influenza a, immune system
• Biological source: Influenza A virus; More
• Total number of polymer chains: 3
• Total formula weight: 84549.43

Authors

Chen, C.,Wang, J.,Wang, W.,Gao, X.,Cui, S.,Jin, Q. (deposition date: 2017-05-09, release date: 2017-11-29, Last modification date: 2024-10-30)

Primary citation

Chen, C.,Liu, L.,Xiao, Y.,Cui, S.,Wang, J.,Jin, Q.
Structural Insight into a Human Neutralizing Antibody against Influenza Virus H7N9
J. Virol., 92:-, 2018

PubMed Abstract: Since its first emergence in East China in early 2013, many cases of avian influenza A H7N9 have been reported. The disease has extended to 22 provinces in mainland China and some surrounding areas. Strategies to combat viral infection are urgently needed. We previously isolated a human monoclonal antibody, HNIgGA6, that neutralized the H7N9 virus both and In this study, we determined the crystal structure of viral hemagglutinin (HA) globular head bound to the fragment antigen-binding region (Fab) of HNIgGA6. The crystal structure shows that the tip of the HNIgGA6 heavy-chain complementarity-determining region 3 (HCDR3) directly interposes into the receptor binding site (RBS) and mimics, in many respects, the interaction of the sialic acid receptor. Three residues at Y98, H183, and E190, which are critical to human cellular receptor binding, are also essential for HNIgGA6 recognition. Meanwhile, dual mutations at V186G and L226Q in RBS were able to disrupt viral HA1 binding with the antibody. Our study provides a better understanding of the mechanism for protective antibody recognition and a sound foundation for the design of therapeutic drugs and vaccines against H7N9 influenza. Neutralization by antibody is one of the most important mechanisms for a host to defend against viral infections. Human-originated antibody HNIgGA6 was generated in response to the natural infectious H7N9 virus and showed potential for use in suppression of H7N9 infection, with possible therapeutic implications. The crystal structure of the HNIgGA6/HA1 complex provided new insight into the protective immune response to H7N9 virus in humans, as well as possibilities for the development of effective H7N9 pandemic vaccines and antiviral molecules.

PubMed: 29212936
DOI: 10.1128/JVI.01850-17

Experimental method

X-RAY DIFFRACTION (1.78 Å)