5XJZ

Structure of DNA1-Ag complex

Summary for 5XJZ

• Entry DOI: 10.2210/pdb5xjz/pdb
• Descriptor: DNA (5'-D(*GP*CP*AP*CP*GP*CP*GP*C)-3'), SILVER ION, SPERMINE, ... (4 entities in total)
• Functional Keywords: dna, ag, base pair
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 5351.13

Authors

Liu, H.H.,Gan, J.H. (deposition date: 2017-05-04, release date: 2017-12-06, Last modification date: 2024-03-27)

Primary citation

Liu, H.H.,Shen, F.S.,Haruehanroengra, P.,Yao, Q.Q.,Cheng, Y.S.,Chen, Y.Q.,Yang, C.,Zhang, J.,Wu, B.X.,Luo, Q.,Cui, R.X.,Li, J.X.,Ma, J.B.,Sheng, J.,Gan, J.H.
A DNA Structure Containing AgI -Mediated G:G and C:C Base Pairs
Angew. Chem. Int. Ed. Engl., 56:9430-9434, 2017

PubMed Abstract: Metal-mediated base pairs have been extensively utilized in many research fields, including genetic-code extension, novel therapeutics development, and nanodevice design. Compared to other cations, Ag is more flexible in pairing with natural base pairs. Herein, we present a DNA structure containing two C-Ag -C pairs and the first reported G-Ag -G pair in a short 8mer DNA strand. This structure not only provides detailed insight into these Ag -mediated base-pairing patterns in DNA, but also represents the first nonhelical DNA structure driven by heavy-metal ions, thus further contributing to the structural diversity of DNA. This unique complex structure is highly sequence-dependent, thus implying functional potentials as a new DNA aptamer that can bind and recognize silver ions. These results not only advance our understanding of the interactions between Ag and nucleobases, but also provide a unique structural component for the rational design of new DNA nanodevices.

PubMed: 28635152
DOI: 10.1002/anie.201704891

Experimental method

X-RAY DIFFRACTION (0.98 Å)