5XH2

Crystal structure of a novel PET hydrolase R103G/S131A mutant in complex with pNP from Ideonella sakaiensis 201-F6

5XH2 の概要

• エントリーDOI: 10.2210/pdb5xh2/pdb
• 関連するPDBエントリー: 5XH3
• 分子名称: Poly(ethylene terephthalate) hydrolase, SULFATE ION, P-NITROPHENOL, ... (4 entities in total)
• 機能のキーワード: poly(ethylene terephthalate) hydrolase, substrate binding, inhibitor, hydrolase
• 由来する生物種: Ideonella sakaiensis (strain 201-F6)
• 細胞内の位置: Secreted : A0A0K8P6T7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27889.76

構造登録者

Han, X.,Liu, W.D.,Zheng, Y.Y.,Chen, C.C.,Guo, R.T. (登録日: 2017-04-19, 公開日: 2017-12-20, 最終更新日: 2023-11-22)

主引用文献

Han, X.,Liu, W.,Huang, J.W.,Ma, J.,Zheng, Y.,Ko, T.P.,Xu, L.,Cheng, Y.S.,Chen, C.C.,Guo, R.T.
Structural insight into catalytic mechanism of PET hydrolase
Nat Commun, 8:2106-2106, 2017

PubMed Abstract: PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.

PubMed: 29235460
DOI: 10.1038/s41467-017-02255-z

実験手法

X-RAY DIFFRACTION (1.2 Å)