5XGR

Structure of the S1 subunit C-terminal domain from bat-derived coronavirus HKU5 spike protein

Summary for 5XGR

• Entry DOI: 10.2210/pdb5xgr/pdb
• Descriptor: Spike protein S1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: mers-cov, batcov hku5, ctd, evolution, viral protein
• Biological source: Bat coronavirus HKU5 (BtCoV)
• Cellular location: Spike protein S2: Virion membrane ; Single-pass type I membrane protein . Spike protein S1: Virion membrane ; Peripheral membrane protein : A3EXD0
• Total number of polymer chains: 8
• Total formula weight: 186540.73

Authors

Xue, H.,Qi, J.,Song, H.,Qihui, W.,Shi, Y.,Gao, G.F. (deposition date: 2017-04-16, release date: 2017-05-10, Last modification date: 2024-10-09)

Primary citation

Han, X.,Qi, J.,Song, H.,Wang, Q.,Zhang, Y.,Wu, Y.,Lu, G.,Yuen, K.Y.,Shi, Y.,Gao, G.F.
Structure of the S1 subunit C-terminal domain from bat-derived coronavirus HKU5 spike protein
Virology, 507:101-109, 2017

PubMed Abstract: Accumulating evidence indicates that MERS-CoV originated from bat coronaviruses (BatCoVs). Previously, we demonstrated that both MERS-CoV and BatCoV HKU4 use CD26 as a receptor, but how the BatCoVs evolved to bind CD26 is an intriguing question. Here, we solved the crystal structure of the S1 subunit C-terminal domain of HKU5 (HKU5-CTD), another BatCoV that is phylogenetically related to MERS-CoV but cannot bind to CD26. We observed that the conserved core subdomain and those of other betacoronaviruses (betaCoVs) have a similar topology of the external subdomain, indicating the same ancestor of lineage C betaCoVs. However, two deletions in two respective loops located in HKU5-CTD result in conformational variations in CD26-binding interface and are responsible for the non-binding of HKU5-CTD to CD26. Combined with sequence variation in the HKU5-CTD receptor binding interface, we propose the necessity for surveilling the mutation in BatCoV HKU5 spike protein in case of bat-to-human interspecies transmission.

PubMed: 28432925
DOI: 10.1016/j.virol.2017.04.016

Experimental method

X-RAY DIFFRACTION (2.1 Å)