5XDQ

Bovine heart cytochrome c oxidase in the fully oxidized state with pH 7.3 at 1.77 angstrom resolution

5XDQ の概要

• エントリーDOI: 10.2210/pdb5xdq/pdb
• 関連するPDBエントリー: 5XDX
• 分子名称: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 7A1, mitochondrial, Cytochrome c oxidase subunit 7B, mitochondrial, ... (29 entities in total)
• 機能のキーワード: oxidoreductase, proton pump, heme protein, respiratory chain
• 由来する生物種: Bos taurus (Bovine); 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 446405.89

構造登録者

Luo, F.J.,Shimada, A.,Hagimoto, N.,Shimada, S.,Shinzawa-Itoh, K.,Yamashita, E.,Yoshikawa, S.,Tsukihara, T. (登録日: 2017-03-29, 公開日: 2017-07-12, 最終更新日: 2023-11-22)

主引用文献

Luo, F.,Shinzawa-Itoh, K.,Hagimoto, K.,Shimada, A.,Shimada, S.,Yamashita, E.,Yoshikawa, S.,Tsukihara, T.
Structure of bovine cytochrome c oxidase crystallized at a neutral pH using a fluorinated detergent.
Acta Crystallogr F Struct Biol Commun, 73:416-422, 2017

PubMed Abstract: Cytochrome c oxidase (CcO) couples proton pumping to O reduction. Its enzymatic activity depends sensitively on pH over a wide range. However, owing to difficulty in crystallizing this protein, X-ray structure analyses of bovine CcO aimed at understanding its reaction mechanism have been conducted using crystals prepared at pH 5.7, which is significantly lower than that in the cell. Here, oxidized CcO at pH 7.3 was crystallized using a fluorinated octyl-maltoside derivative, and the structure was determined at 1.77 Å resolution. No structural differences between crystals obtained at the neutral pH and the acidic pH were detected within the molecules. On the other hand, some differences in intermolecular interactions were detected between the two types of crystal. The influence of pH on the molecular surface is likely to contribute to the pH dependency of the aerobic oxidation of ferrocytochrome c.

PubMed: 28695851
DOI: 10.1107/S2053230X17008834

実験手法

X-RAY DIFFRACTION (1.77 Å)