5X8F

Ternary complex structure of a double mutant I454RA456K of o-Succinylbenzoate CoA Synthetase (MenE) from Bacillus Subtilis bound with AMP and its product analogue OSB-NCoA at 1.76 angstrom

Summary for 5X8F

Entry DOI	10.2210/pdb5x8f/pdb
Related	5X8G
Descriptor	2-succinylbenzoate--CoA ligase, o-succinylbenzoyl-N-coenzyme A, ADENOSINE MONOPHOSPHATE, ... (8 entities in total)
Functional Keywords	adenylate-forming enzyme, acyl-coa synthetase, thioester conformation, coa, pantetheine tunnel, adp binding subsite, domain alternation, large conformational change, inter-domain linker, ligase
Biological source	Bacillus subtilis subsp. subtilis str. 168
Total number of polymer chains	4
Total formula weight	223682.85
Authors	Chen, Y.,Guo, Z. (deposition date: 2017-03-02, release date: 2017-06-07, Last modification date: 2023-11-22)
Primary citation	Chen, Y.,Li, T.L.,Lin, X.,Li, X.,Li, X.D.,Guo, Z. Crystal structure of the thioesterification conformation of Bacillus subtilis o-succinylbenzoyl-CoA synthetase reveals a distinct substrate-binding mode J. Biol. Chem., 292:12296-12310, 2017 Cited by PubMed: 28559280 DOI: 10.1074/jbc.M117.790410 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.763 Å)

Structure validation