5X29

NMR structure of the SARS Coronavirus E protein pentameric ion channel

5X29 の概要

• エントリーDOI: 10.2210/pdb5x29/pdb
• 関連するPDBエントリー: 2MM4
• NMR情報: BMRB: 36049
• 分子名称: Envelope small membrane protein (1 entity in total)
• 機能のキーワード: membrane protein, viral protein, envelope protein, ion channel, pentamer
• 由来する生物種: Human SARS coronavirus (SARS-CoV)
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 45017.70

構造登録者

Torres, J.,Surya, W.,Li, Y. (登録日: 2017-01-31, 公開日: 2017-06-07, 最終更新日: 2024-05-15)

主引用文献

Surya, W.,Li, Y.,Torres, J.
Structural model of the SARS coronavirus E channel in LMPG micelles
Biochim. Biophys. Acta, 1860:1309-1317, 2018

PubMed Abstract: Coronaviruses (CoV) cause common colds in humans, but are also responsible for the recent Severe Acute, and Middle East, respiratory syndromes (SARS and MERS, respectively). A promising approach for prevention are live attenuated vaccines (LAVs), some of which target the envelope (E) protein, which is a small membrane protein that forms ion channels. Unfortunately, detailed structural information is still limited for SARS-CoV E, and non-existent for other CoV E proteins. Herein, we report a structural model of a SARS-CoV E construct in LMPG micelles with, for the first time, unequivocal intermolecular NOEs. The model corresponding to the detergent-embedded region is consistent with previously obtained orientational restraints obtained in lipid bilayers and in vivo escape mutants. The C-terminal domain is mostly α-helical, and extramembrane intermolecular NOEs suggest interactions that may affect the TM channel conformation.

PubMed: 29474890
DOI: 10.1016/j.bbamem.2018.02.017

実験手法

SOLUTION NMR