5WYD

Structural of Pseudomonas aeruginosa DspI

Summary for 5WYD

• Entry DOI: 10.2210/pdb5wyd/pdb
• Related: 5WYB 5WYC
• Descriptor: Probable enoyl-CoA hydratase/isomerase, PENTANEDIAL, ISOPROPYL ALCOHOL, ... (6 entities in total)
• Functional Keywords: crotonase, enoyl-coenzyme a (coa) hydratase/isomeras, diffusible signal factor synthesis, isomerase
• Biological source: Pseudomonas aeruginosa PAO1
• Total number of polymer chains: 6
• Total formula weight: 187054.80

Authors

Liu, L.,Peng, C.,Li, T.,Li, C.,He, L.,Song, Y.,Zhu, Y.,Shen, Y.,Bao, R. (deposition date: 2017-01-12, release date: 2018-01-31, Last modification date: 2023-11-22)

Primary citation

Liu, L.,Li, T.,Cheng, X.J.,Peng, C.T.,Li, C.C.,He, L.H.,Ju, S.M.,Wang, N.Y.,Ye, T.H.,Lian, M.,Xiao, Q.J.,Song, Y.J.,Zhu, Y.B.,Yu, L.T.,Wang, Z.L.,Bao, R.
Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.
Sci Rep, 8:3928-3928, 2018

PubMed Abstract: DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa (P. aeruginosa). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of dspI results in a remarkable decreased in the pyoverdine production, flagella-dependent swarming motility, and biofilm dispersion as well as attenuated virulence in P. aeruginosa PA14. This study thus unravels the mechanism of DspI in diffusible signal factor (DSF) CDA biosynthesis, providing vital information for developing inhibitors that interfere with DSF associated pathogenicity in P. aeruginosa.

PubMed: 29500457
DOI: 10.1038/s41598-018-22300-1

Experimental method

X-RAY DIFFRACTION (2.101 Å)