5WXH

Crystal structure of TAF3 PHD finger bound to H3K4me3

5WXH の概要

• エントリーDOI: 10.2210/pdb5wxh/pdb
• 関連するPDBエントリー: 5WXG
• 分子名称: Transcription initiation factor TFIID subunit 3, Histone H3K4me3, ZINC ION, ... (4 entities in total)
• 機能のキーワード: taf3, histone h3, hydrolase
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : Q5VWG9
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 16480.15

構造登録者

Zhao, S.,Huang, J.,Li, H. (登録日: 2017-01-07, 公開日: 2017-08-16, 最終更新日: 2017-09-13)

主引用文献

Zhao, S.,Yang, M.,Zhou, W.,Zhang, B.,Cheng, Z.,Huang, J.,Zhang, M.,Wang, Z.,Wang, R.,Chen, Z.,Zhu, J.,Li, H.
Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology
Proc. Natl. Acad. Sci. U.S.A., 114:E7245-E7254, 2017

PubMed Abstract: Chemical modifications on histones and DNA/RNA constitute a fundamental mechanism for epigenetic regulation. These modifications often function as docking marks to recruit or stabilize cognate "reader" proteins. So far, a platform for quantitative and high-throughput profiling of the epigenetic interactome is urgently needed but still lacking. Here, we report a 3D-carbene chip-based surface plasmon resonance imaging (SPRi) technology for this purpose. The 3D-carbene chip is suitable for immobilizing versatile biomolecules (e.g., peptides, antibody, DNA/RNA) and features low nonspecific binding, random yet function-retaining immobilization, and robustness for reuses. We systematically profiled binding kinetics of 1,000 histone "reader-mark" pairs on a single 3D-carbene chip and validated two recognition events by calorimetric and structural studies. Notably, a discovery on H3K4me3 recognition by the DNA mismatch repair protein MSH6 in suggests a mechanism of H3K4me3-mediated DNA damage repair in plant.

PubMed: 28808021
DOI: 10.1073/pnas.1704155114

実験手法

X-RAY DIFFRACTION (1.297 Å)