5WV7

Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4A resolution

Summary for 5WV7

• Entry DOI: 10.2210/pdb5wv7/pdb
• Descriptor: DNA (5'-D(*CP*CP*GP*GP*GP*GP*TP*AP*CP*CP*CP*CP*GP*G)-3') (2 entities in total)
• Functional Keywords: a-dna duplex, tetragonal space group, co-crystallization with barium chloride., dna
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 8565.54

Authors

Karthik, S.,Thirugnanasambandam, A.,Mandal, P.K.,Gautham, N. (deposition date: 2016-12-22, release date: 2017-05-24, Last modification date: 2023-11-22)

Primary citation

Karthik, S.,Thirugnanasambandam, A.,Mandal, P.K.,Gautham, N.
Crystal structure of d(CCGGGGTACCCCGG)2 at 1.4 angstrom resolution.
Acta Crystallogr F Struct Biol Commun, 73:259-265, 2017

PubMed Abstract: The X-ray crystal structure of the DNA tetradecamer sequence d(CCGGGGTACCCCGG) is reported at 1.4 Å resolution in the tetragonal space group P422. The sequence was designed to fold as a four-way junction. However, it forms an A-type double helix in the presence of barium chloride. The metal ion could not be identified in the electron-density map. The crystallographic asymmetric unit consists of one A-type double helix with 12 base pairs per turn, in contrast to 11 base pairs per turn for canonical A-DNA. A large number of solvent molecules have been identified in both the grooves of the duplex and around the backbone phosphate groups.

PubMed: 28471357
DOI: 10.1107/S2053230X17004770

Experimental method

X-RAY DIFFRACTION (1.406 Å)