5WQG
Structure of fungal meroterpenoid isomerase Trt14 complexed with terretonin D
Summary for 5WQG
| Entry DOI | 10.2210/pdb5wqg/pdb |
| Related | 5WQF 5WQH 5WQI |
| Descriptor | Isomerase trt14, CALCIUM ION, methyl (2S,4aR,4bS,5S,6aS,10aS,10bS,12aS)-2,4b,7,7,10a,12a-hexamethyl-12-methylidene-5-oxidanyl-1,4,6,8-tetrakis(oxidanylidene) -5,6a,9,10,10b,11-hexahydro-4aH-naphtho[1,2-h]isochromene-2-carboxylate, ... (4 entities in total) |
| Functional Keywords | isomerase, meroterpenoid, terretonin |
| Biological source | Aspergillus terreus NIH 2624 |
| Total number of polymer chains | 6 |
| Total formula weight | 111605.49 |
| Authors | Mori, T.,Matsuda, Y.,Abe, I. (deposition date: 2016-11-26, release date: 2017-07-26, Last modification date: 2023-11-08) |
| Primary citation | Mori, T.,Iwabuchi, T.,Hoshino, S.,Wang, H.,Matsuda, Y.,Abe, I. Molecular basis for the unusual ring reconstruction in fungal meroterpenoid biogenesis Nat. Chem. Biol., 13:1066-1073, 2017 Cited by PubMed Abstract: Trt14 from Aspergillus terreus is involved in unusual skeletal reconstruction during the biosynthesis of the fungal meroterpenoid terretonin. Detailed in vitro characterization revealed that this novel multifunctional enzyme catalyzes not only the D-ring expansion via intramolecular methoxy rearrangement, but also the hydrolysis of the expanded D-ring. The X-ray crystal structures of Trt14, in complex with substrate or product, and two Trt14 homologs, AusH and PrhC from Aspergillus nidulans and Penicillium brasilianum, respectively, indicated similar overall structures to those of the NTF2-like superfamily of enzymes, despite lacking sequence and functional similarities. Moreover, we gained structural insight into the mechanism of the Trt14-catalyzed ring reconstruction from the in-crystal enzyme reaction and site-directed mutagenesis to show that this reaction involves sequential ester bond cleavage and formation. Structural comparison of Trt14 and its homologs suggests that the enzymes in this new superfamily employ similar acid-base chemistry to diversify the molecular architecture of fungal meroterpenoids. PubMed: 28759016DOI: 10.1038/nchembio.2443 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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