5WKO

Crystal structure of antibody 27F3 recognizing the HA from A/California/04/2009 (H1N1) influenza virus

Summary for 5WKO

• Entry DOI: 10.2210/pdb5wko/pdb
• Descriptor: Antibody 27F3 heavy chain, Antibody 27F3 light chain, Hemagglutinin, ... (4 entities in total)
• Functional Keywords: 27f3 h1 ha a/california/04/2009, antiviral protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 24
• Total formula weight: 623364.73

Authors

Wilson, I.A.,Lang, S.,Zhu, X. (deposition date: 2017-07-25, release date: 2017-10-25, Last modification date: 2024-05-15)

Primary citation

Lang, S.,Xie, J.,Zhu, X.,Wu, N.C.,Lerner, R.A.,Wilson, I.A.
Antibody 27F3 Broadly Targets Influenza A Group 1 and 2 Hemagglutinins through a Further Variation in VH1-69 Antibody Orientation on the HA Stem.
Cell Rep, 20:2935-2943, 2017

PubMed Abstract: Antibodies that target both group 1 and group 2 influenza A viruses are valuable for therapeutic and vaccine development, but only a few have been reported to date. Here, we describe a new V1-69 antibody 27F3 that broadly recognizes heterosubtypic hemagglutinins (HAs) from both group 1 and group 2 influenza A viruses. Structural characterization of 27F3 Fab with A/California/04/2009 (H1N1) hemagglutinin illustrates that 27F3 shares the key binding features observed in other V1-69 antibodies to the HA stem. Compared to other V1-69 antibodies, the 27F3 V domain interacts with the HA stem in a distinct orientation, which alters its epitope and may have influenced its breadth. The diverse rotations of V1-69 antibodies on the HA stem epitope highlight the different ways that this antibody family can evolve to broadly neutralize influenza A viruses. These results have important implications for understanding how to elicit broad antibody responses against influenza virus.

PubMed: 28930686
DOI: 10.1016/j.celrep.2017.08.084

Experimental method

X-RAY DIFFRACTION (3.492 Å)