5WKO

Crystal structure of antibody 27F3 recognizing the HA from A/California/04/2009 (H1N1) influenza virus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	Antibody 27F3 heavy chain	polymer	225	23817.7	6	UniProt (Q6N089) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (Human)
2	B, D, F, H, J... (B, D, F, H, J...)	Antibody 27F3 light chain	polymer	213	23140.7	6	UniProt (Q9UL78) UniProt (Q6P5S8) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (Human)
3	M, N, O, S, T... (M, N, O, S, T...)	Hemagglutinin	polymer	331	36729.4	6	UniProt (C3W5S1) Pfam (PF00509)	Influenza A virus
4	P, Q, R, V, W... (P, Q, R, V, W...)	Hemagglutinin	polymer	177	20206.3	6	UniProt (A0A023ZYH9) Pfam (PF00509) UniProt (by SIFTS) (C5MVT7)	Influenza A virus (A/Uganda/MUWRP-176/2010(H1N1))

Sequence modifications

A, C, E, G, I, K: 1 - 105 (PDB: 5WKO)
A, C, E, G, I, K: 106 - 215 (UniProt: Q6N089)
B, D, F, H, J, L: 1 - 103 (UniProt: Q9UL78)

PDB	External Database	Details
Thr 76	Ser 77	conflict
Phe 83	Cys 84	conflict
Val 92	Gly 93	conflict
Thr 94	Ser 95	conflict

B, D, F, H, J, L: 104 - 212 (UniProt: Q6P5S8)
M, N, O, S, T, U: 11 - 329 (UniProt: C3W5S1)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag

P, Q, R, V, W, X: 1 - 174 (UniProt: A0A023ZYH9)

PDB	External Database	Details
Gly 47	Glu 391	engineered mutation
Ser 175	-	expression tag
Gly 176	-	expression tag
Arg 177	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	623364.7
All*	Total formula weight	623364.7

*Water molecules are not included.