5WDD

Crystal structure of chicken BOK

5WDD の概要

• エントリーDOI: 10.2210/pdb5wdd/pdb
• 分子名称: Bcl-2-related ovarian killer protein, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: bcl-2, apoptosis, pro-apoptotic, cell death
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37098.86

構造登録者

Cowan, A.D.,Colman, P.M.,Czabotar, P.E. (登録日: 2017-07-04, 公開日: 2018-02-28, 最終更新日: 2023-10-04)

主引用文献

Ke, F.F.S.,Vanyai, H.K.,Cowan, A.D.,Delbridge, A.R.D.,Whitehead, L.,Grabow, S.,Czabotar, P.E.,Voss, A.K.,Strasser, A.
Embryogenesis and Adult Life in the Absence of Intrinsic Apoptosis Effectors BAX, BAK, and BOK.
Cell, 173:1217-1230.e17, 2018

PubMed Abstract: Intrinsic apoptosis, reliant on BAX and BAK, has been postulated to be fundamental for morphogenesis, but its precise contribution to this process has not been fully explored in mammals. Our structural analysis of BOK suggests close resemblance to BAX and BAK structures. Notably, BokBaxBak animals exhibited more severe defects and died earlier than BaxBak mice, implying that BOK has overlapping roles with BAX and BAK during developmental cell death. By analyzing BokBaxBak triple-knockout mice whose cells are incapable of undergoing intrinsic apoptosis, we identified tissues that formed well without this process. We provide evidence that necroptosis, pyroptosis, or autophagy does not substantially substitute for the loss of apoptosis. Albeit very rare, unexpected attainment of adult BokBaxBak mice suggests that morphogenesis can proceed entirely without apoptosis mediated by these proteins and possibly without cell death in general.

PubMed: 29775594
DOI: 10.1016/j.cell.2018.04.036

実験手法

X-RAY DIFFRACTION (1.799 Å)