5W8N

Lipid A Disaccharide Synthase (LpxB)-6 solubilizing mutations

5W8N の概要

• エントリーDOI: 10.2210/pdb5w8n/pdb
• 関連するPDBエントリー: 5W8S 5W8X
• 分子名称: Lipid-A-disaccharide synthase (2 entities in total)
• 機能のキーワード: glycosyltransferase b, rossmann-like, c-terminal swap, dimer, lipid a disaccharide synthase, raetz pathway, lipid a synthesis pathway, lipiopolysaccharide synthesis, transferase
• 由来する生物種: Escherichia coli BL21(DE3)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42325.99

構造登録者

Bohl, T.E.,Aihara, H.,Shi, K.,Lee, J.K. (登録日: 2017-06-22, 公開日: 2018-01-31, 最終更新日: 2024-03-13)

主引用文献

Bohl, T.E.,Shi, K.,Lee, J.K.,Aihara, H.
Crystal structure of lipid A disaccharide synthase LpxB from Escherichia coli.
Nat Commun, 9:377-377, 2018

PubMed Abstract: Most Gram-negative bacteria are surrounded by a glycolipid called lipopolysaccharide (LPS), which forms a barrier to hydrophobic toxins and, in pathogenic bacteria, is a virulence factor. During LPS biosynthesis, a membrane-associated glycosyltransferase (LpxB) forms a tetra-acylated disaccharide that is further acylated to form the membrane anchor moiety of LPS. Here we solve the structure of a soluble and catalytically competent LpxB by X-ray crystallography. The structure reveals that LpxB has a glycosyltransferase-B family fold but with a highly intertwined, C-terminally swapped dimer comprising four domains. We identify key catalytic residues with a product, UDP, bound in the active site, as well as clusters of hydrophobic residues that likely mediate productive membrane association or capture of lipidic substrates. These studies provide the basis for rational design of antibiotics targeting a crucial step in LPS biosynthesis.

PubMed: 29371662
DOI: 10.1038/s41467-017-02712-9

実験手法

X-RAY DIFFRACTION (2.02 Å)