5W8N
Lipid A Disaccharide Synthase (LpxB)-6 solubilizing mutations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 67.784, 67.784, 154.503 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.570 - 2.020 |
R-factor | 0.1885 |
Rwork | 0.187 |
R-free | 0.21290 |
Structure solution method | SAD |
Data reduction software | XDS (November 1, 2017) |
Data scaling software | Aimless (0.5.23) |
Phasing software | PHENIX (v1.11.1_2575) |
Refinement software | PHENIX (v1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.570 | 2.070 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.112 | 1.686 |
Rpim | 0.047 | 0.717 |
Number of reflections | 27746 | 1958 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 99.6 | 97 |
Redundancy | 6.5 | 6.1 |
CC(1/2) | 0.997 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 292 | 40 % PEG 4000, 0.1 M Tris-HCl pH 8.6, 0.8 M LiCl combined 1:1 with 8 g/L protein in 0.3 M NaCl, 20 mM Tris-HCl pH 7.5, 5% glycerol, 5 mM DTT with 100 mM trimethylammonium chloride additive used at 10% of drop volume ie. 10 mM final concentration |