5W65

RNA polymerase I Initial Transcribing Complex State 2

Summary for 5W65

• Entry DOI: 10.2210/pdb5w65/pdb
• EMDB information: 8771 8772 8773 8774 8775 8776 8777
• Descriptor: DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (21 entities in total)
• Functional Keywords: rna polymerase i core factor transcription, transcription
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 20
• Total formula weight: 846275.53

Authors

Han, Y.,He, Y. (deposition date: 2017-06-16, release date: 2017-08-02, Last modification date: 2024-12-25)

Primary citation

Han, Y.,Yan, C.,Nguyen, T.H.D.,Jackobel, A.J.,Ivanov, I.,Knutson, B.A.,He, Y.
Structural mechanism of ATP-independent transcription initiation by RNA polymerase I.
Elife, 6:-, 2017

PubMed Abstract: Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Pol I-CF-DNA to 3.8 Å resolution using single-particle cryo-electron microscopy. The structure reveals a bipartite architecture of Core Factor and its recognition of the promoter from -27 to -16. Core Factor's intrinsic mobility correlates well with different conformational states of the Pol I cleft, in addition to the stabilization of either Rrn7 N-terminal domain near Pol I wall or the tandem winged helix domain of A49 at a partially overlapping location. Comparison of the three states in this study with the Pol II system suggests that a ratchet motion of the Core Factor-DNA sub-complex at upstream facilitates promoter melting in an ATP-independent manner, distinct from a DNA translocase actively threading the downstream DNA in the Pol II PIC.

PubMed: 28623663
DOI: 10.7554/eLife.27414

Experimental method

ELECTRON MICROSCOPY (4.3 Å)