5W4W

Identification and Profiling of a Selective and Brain Penetrant Radioligand for In Vivo Target Occupancy Measurement of Casein Kinase 1 (CK1) Inhibitors

Summary for 5W4W

• Entry DOI: 10.2210/pdb5w4w/pdb
• Descriptor: Casein kinase I isoform delta, 4-[3-(4-fluorophenyl)-1-methyl-1H-pyrazol-4-yl]-6-methyl-6,7-dihydro-5H-pyrrolo[3,4-b]pyridin-5-one, SULFATE ION, ... (4 entities in total)
• Functional Keywords: kinase, inhibitor, transferase-inhibitor complex, transferase/inhibitor
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm: P48730
• Total number of polymer chains: 4
• Total formula weight: 156879.18

Authors

Liu, S. (deposition date: 2017-06-13, release date: 2017-06-28, Last modification date: 2024-03-13)

Primary citation

Wager, T.T.,Galatsis, P.,Chandrasekaran, R.Y.,Butler, T.W.,Li, J.,Zhang, L.,Mente, S.,Subramanyam, C.,Liu, S.,Doran, A.C.,Chang, C.,Fisher, K.,Grimwood, S.,Hedde, J.R.,Marconi, M.,Schildknegt, K.
Identification and Profiling of a Selective and Brain Penetrant Radioligand for in Vivo Target Occupancy Measurement of Casein Kinase 1 (CK1) Inhibitors.
ACS Chem Neurosci, 8:1995-2004, 2017

PubMed Abstract: To enable the clinical development of our CNS casein kinase 1 delta/epsilon (CK1δ/ε) inhibitor project, we investigated the possibility of developing a CNS positron emission tomography (PET) radioligand. For this effort, we focused our design and synthesis efforts on the initial CK1δ/ε inhibitor HTS hits with the goal of identifying a compound that would fulfill a set of recommended PET ligand criteria. We identified [H]PF-5236216 (9) as a tool ligand that meets most of the key CNS PET attributes including high CNS MPO PET desirability score and kinase selectivity, CNS penetration, and low nonspecific binding. We further used [H]-9 to determine the binding affinity for PF-670462, a literature CK1δ/ε inhibitor tool compound. Lastly, [H]-9 was used to measure in vivo target occupancy (TO) of PF-670462 in mouse and correlated TO with CK1δ/ε in vivo pharmacology (circadian rhythm modulation).

PubMed: 28609096
DOI: 10.1021/acschemneuro.7b00155

Experimental method

X-RAY DIFFRACTION (1.99 Å)