5W1S

X-ray crystal structure of Escherichia coli RNA polymerase and TraR complex

5W1S の概要

• エントリーDOI: 10.2210/pdb5w1s/pdb
• 関連するPDBエントリー: 5VSW 5W20
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (8 entities in total)
• 機能のキーワード: rna polymerase, trar, transferase
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 938561.42

構造登録者

Murakami, K.S.,Molodtsov, V. (登録日: 2017-06-04, 公開日: 2017-06-14, 最終更新日: 2023-10-04)

主引用文献

Molodtsov, V.,Sineva, E.,Zhang, L.,Huang, X.,Cashel, M.,Ades, S.E.,Murakami, K.S.
Allosteric Effector ppGpp Potentiates the Inhibition of Transcript Initiation by DksA.
Mol. Cell, 69:828-839.e5, 2018

PubMed Abstract: DksA and ppGpp are the central players in the stringent response and mediate a complete reprogramming of the transcriptome. A major component of the response is a reduction in ribosome synthesis, which is accomplished by the synergistic action of DksA and ppGpp bound to RNA polymerase (RNAP) inhibiting transcription of rRNAs. Here, we report the X-ray crystal structures of Escherichia coli RNAP in complex with DksA alone and with ppGpp. The structures show that DksA accesses the template strand at the active site and the downstream DNA binding site of RNAP simultaneously and reveal that binding of the allosteric effector ppGpp reshapes the RNAP-DksA complex. The structural data support a model for transcriptional inhibition in which ppGpp potentiates the destabilization of open complexes by DksA. This work establishes a structural basis for understanding the pleiotropic effects of DksA and ppGpp on transcriptional regulation in proteobacteria.

PubMed: 29478808
DOI: 10.1016/j.molcel.2018.01.035

実験手法

X-RAY DIFFRACTION (3.805 Å)