5VY5

Rabbit muscle aldolase using 200keV

Summary for 5VY5

Entry DOI	10.2210/pdb5vy5/pdb
EMDB information	8743
Descriptor	Fructose-bisphosphate aldolase A (1 entity in total)
Functional Keywords	glycolytic enzyme, lyase
Biological source	Oryctolagus cuniculus (Rabbit)
Total number of polymer chains	4
Total formula weight	157054.69
Authors	Herzik Jr., M.A.,Wu, M.,Lander, G.C. (deposition date: 2017-05-24, release date: 2017-06-14, Last modification date: 2024-03-13)
Primary citation	Herzik, M.A.,Wu, M.,Lander, G.C. Achieving better-than-3- angstrom resolution by single-particle cryo-EM at 200 keV. Nat. Methods, 14:1075-1078, 2017 Cited by PubMed Abstract: Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules. PubMed: 28991891 DOI: 10.1038/nmeth.4461 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.6 Å)

Structure validation