5VPS

Crystal structure of an SDR from Burkholderia ambifaria in complex with NADPH with a TCEP adduct

Summary for 5VPS

• Entry DOI: 10.2210/pdb5vps/pdb
• Descriptor: Short-chain dehydrogenase/reductase SDR, [(4~{S})-3-aminocarbonyl-1-[(2~{R},3~{R},4~{S},5~{R})-5-[[[[(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]piperidin-4-yl]-tris(3-hydroxy-3-oxopropyl)phosphanium, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: sgcid, short chain, dehydrogenase, reductase, nadp, nadph, tcep, structural genomics, seattle structural genomics center for infectious disease, ssgcid, oxidoreductase
• Biological source: Burkholderia ambifaria (strain MC40-6)
• Total number of polymer chains: 1
• Total formula weight: 28767.20

Authors

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2017-05-05, release date: 2017-05-24, Last modification date: 2023-10-04)

• Primary citation: Patel, S.M.,Smith, T.G.,Morton, M.,Stiers, K.M.,Seravalli, J.,Mayclin, S.J.,Edwards, T.E.,Tanner, J.J.,Becker, D.F.; Cautionary Tale of Using Tris(alkyl)phosphine Reducing Agents with NAD+-Dependent Enzymes.; Biochemistry, 2020; PubMed: 32841567; DOI: 10.1021/acs.biochem.0c00490

Experimental method

X-RAY DIFFRACTION (1.45 Å)