5VPS

Crystal structure of an SDR from Burkholderia ambifaria in complex with NADPH with a TCEP adduct

5VPS の概要

• エントリーDOI: 10.2210/pdb5vps/pdb
• 分子名称: Short-chain dehydrogenase/reductase SDR, [(4~{S})-3-aminocarbonyl-1-[(2~{R},3~{R},4~{S},5~{R})-5-[[[[(2~{R},3~{R},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-oxidanyl-4-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]piperidin-4-yl]-tris(3-hydroxy-3-oxopropyl)phosphanium, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: sgcid, short chain, dehydrogenase, reductase, nadp, nadph, tcep, structural genomics, seattle structural genomics center for infectious disease, ssgcid, oxidoreductase
• 由来する生物種: Burkholderia ambifaria (strain MC40-6)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28767.20

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2017-05-05, 公開日: 2017-05-24, 最終更新日: 2023-10-04)

主引用文献

Patel, S.M.,Smith, T.G.,Morton, M.,Stiers, K.M.,Seravalli, J.,Mayclin, S.J.,Edwards, T.E.,Tanner, J.J.,Becker, D.F.
Cautionary Tale of Using Tris(alkyl)phosphine Reducing Agents with NAD+-Dependent Enzymes.
Biochemistry, 2020

PubMed Abstract: Protein biochemistry protocols typically include disulfide bond reducing agents to guard against unwanted thiol oxidation and protein aggregation. Commonly used disulfide bond reducing agents include dithiothreitol, β-mercaptoethanol, glutathione, and the tris(alkyl)phosphine compounds tris(2-carboxyethyl)phosphine (TCEP) and tris(3-hydroxypropyl)phosphine (THPP). While studying the catalytic activity of the NAD(P)H-dependent enzyme Δ-pyrroline-5-carboxylate reductase, we unexpectedly observed a rapid non-enzymatic chemical reaction between NAD and the reducing agents TCEP and THPP. The product of the reaction exhibits a maximum ultraviolet absorbance peak at 334 nm and forms with an apparent association rate constant of 231-491 M s. The reaction is reversible, and nuclear magnetic resonance characterization (H, C, and P) of the product revealed a covalent adduct between the phosphorus of the tris(alkyl)phosphine reducing agent and the C4 atom of the nicotinamide ring of NAD. We also report a 1.45 Å resolution crystal structure of short-chain dehydrogenase/reductase with the NADP-TCEP reaction product bound in the cofactor binding site, which shows that the adduct can potentially inhibit enzymes. These findings serve to caution researchers when using TCEP or THPP in experimental protocols with NAD(P). Because NAD(P)-dependent oxidoreductases are widespread in nature, our results may be broadly relevant.

PubMed: 32841567
DOI: 10.1021/acs.biochem.0c00490

実験手法

X-RAY DIFFRACTION (1.45 Å)