5VOI

X-ray crystal structure of bacterial RNA polymerase and pyrG promoter complex

Summary for 5VOI

• Entry DOI: 10.2210/pdb5voi/pdb
• Related: 5VO8
• Descriptor: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
• Functional Keywords: thermus thermophilus, rna polymerase, reiterative transcription, holoenzyme, transcription-dna-rna complex, transcription/dna/rna
• Biological source: Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039); More
• Total number of polymer chains: 8
• Total formula weight: 441968.10

Authors

Murakami, K.S.,Shin, Y.,Turnbough Jr, C.L.,Molodtsov, V. (deposition date: 2017-05-02, release date: 2017-05-24, Last modification date: 2024-10-23)

Primary citation

Murakami, K.S.,Shin, Y.,Turnbough, C.L.,Molodtsov, V.
X-ray crystal structure of a reiterative transcription complex reveals an atypical RNA extension pathway.
Proc. Natl. Acad. Sci. U.S.A., 114:8211-8216, 2017

PubMed Abstract: Reiterative transcription is a noncanonical form of RNA synthesis in which a nucleotide specified by a single base in the DNA template is repetitively added to the nascent transcript. Here we determined the crystal structure of an RNA polymerase, the bacterial enzyme from , engaged in reiterative transcription during transcription initiation at a promoter resembling the promoter of The structure reveals that the reiterative transcript detours from the dedicated RNA exit channel and extends toward the main channel of the enzyme, thereby allowing RNA extension without displacement of the promoter recognition σ-factor. Nascent transcripts containing reiteratively added G residues are eventually extended by nonreiterative transcription, revealing an atypical pathway for the formation of a transcription elongation complex.

PubMed: 28652344
DOI: 10.1073/pnas.1702741114

Experimental method

X-RAY DIFFRACTION (2.8 Å)