5VN8

Cryo-EM model of B41 SOSIP.664 in complex with fragment antigen binding variable domain of b12

5VN8 の概要

• エントリーDOI: 10.2210/pdb5vn8/pdb
• 関連するPDBエントリー: 5vn3
• EMDBエントリー: 8713 8714 8715 8716 8717
• 分子名称: Envelope glycoprotein gp160, b12 Fab heavy chain, b12 Fab light chain, ... (8 entities in total)
• 機能のキーワード: neutralizing antibody, hiv-1, envelope glycoprotein, receptor binding site, immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 394772.69

構造登録者

Ozorowski, G.,Pallesen, J.,Ward, A.B.,Cottrell, C.A. (登録日: 2017-04-28, 公開日: 2017-07-12, 最終更新日: 2024-12-25)

主引用文献

Ozorowski, G.,Pallesen, J.,de Val, N.,Lyumkis, D.,Cottrell, C.A.,Torres, J.L.,Copps, J.,Stanfield, R.L.,Cupo, A.,Pugach, P.,Moore, J.P.,Wilson, I.A.,Ward, A.B.
Open and closed structures reveal allostery and pliability in the HIV-1 envelope spike.
Nature, 547:360-363, 2017

PubMed Abstract: For many enveloped viruses, binding to a receptor(s) on a host cell acts as the first step in a series of events culminating in fusion with the host cell membrane and transfer of genetic material for replication. The envelope glycoprotein (Env) trimer on the surface of HIV is responsible for receptor binding and fusion. Although Env can tolerate a high degree of mutation in five variable regions (V1-V5), and also at N-linked glycosylation sites that contribute roughly half the mass of Env, the functional sites for recognition of receptor CD4 and co-receptor CXCR4/CCR5 are conserved and essential for viral fitness. Soluble SOSIP Env trimers are structural and antigenic mimics of the pre-fusion native, surface-presented Env, and are targets of broadly neutralizing antibodies. Thus, they are attractive immunogens for vaccine development. Here we present high-resolution cryo-electron microscopy structures of subtype B B41 SOSIP Env trimers in complex with CD4 and antibody 17b, or with antibody b12, at resolutions of 3.7 Å and 3.6 Å, respectively. We compare these to cryo-electron microscopy reconstructions of B41 SOSIP Env trimers with no ligand or in complex with either CD4 or the CD4-binding-site antibody PGV04 at 5.6 Å, 5.2 Å and 7.4 Å resolution, respectively. Consequently, we present the most complete description yet, to our knowledge, of the CD4-17b-induced intermediate and provide the molecular basis of the receptor-binding-induced conformational change required for HIV-1 entry into host cells. Both CD4 and b12 induce large, previously uncharacterized conformational rearrangements in the gp41 subunits, and the fusion peptide becomes buried in a newly formed pocket. These structures provide key details on the biological function of the type I viral fusion machine from HIV-1 as well as new templates for inhibitor design.

PubMed: 28700571
DOI: 10.1038/nature23010

実験手法

ELECTRON MICROSCOPY (3.6 Å)