5VJM

Crystal structure of H7 hemagglutinin mutant (V186K, K193T, G228S) from the influenza virus A/Shanghai/2/2013 (H7N9) with LSTa

5VJM の概要

• エントリーDOI: 10.2210/pdb5vjm/pdb
• 関連するPDBエントリー: 5VJK 5VJL
• 分子名称: Hemagglutinin HA1, Hemagglutinin HA2, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: influenza virus, hemagglutinin, mutant, receptor specificity, viral protein
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57452.03

構造登録者

Zhu, X.,Wilson, I.A. (登録日: 2017-04-19, 公開日: 2017-05-24, 最終更新日: 2024-10-23)

主引用文献

de Vries, R.P.,Peng, W.,Grant, O.C.,Thompson, A.J.,Zhu, X.,Bouwman, K.M.,de la Pena, A.T.T.,van Breemen, M.J.,Ambepitiya Wickramasinghe, I.N.,de Haan, C.A.M.,Yu, W.,McBride, R.,Sanders, R.W.,Woods, R.J.,Verheije, M.H.,Wilson, I.A.,Paulson, J.C.
Three mutations switch H7N9 influenza to human-type receptor specificity.
PLoS Pathog., 13:e1006390-e1006390, 2017

PubMed Abstract: The avian H7N9 influenza outbreak in 2013 resulted from an unprecedented incidence of influenza transmission to humans from infected poultry. The majority of human H7N9 isolates contained a hemagglutinin (HA) mutation (Q226L) that has previously been associated with a switch in receptor specificity from avian-type (NeuAcα2-3Gal) to human-type (NeuAcα2-6Gal), as documented for the avian progenitors of the 1957 (H2N2) and 1968 (H3N2) human influenza pandemic viruses. While this raised concern that the H7N9 virus was adapting to humans, the mutation was not sufficient to switch the receptor specificity of H7N9, and has not resulted in sustained transmission in humans. To determine if the H7 HA was capable of acquiring human-type receptor specificity, we conducted mutation analyses. Remarkably, three amino acid mutations conferred a switch in specificity for human-type receptors that resembled the specificity of the 2009 human H1 pandemic virus, and promoted binding to human trachea epithelial cells.

PubMed: 28617868
DOI: 10.1371/journal.ppat.1006390

実験手法

X-RAY DIFFRACTION (2.909 Å)